The Department of Agriculture in Thailand is using tissue culture techniques to produce disease-free and non-toxic betel nut trees, which are important for their medicinal and cosmetic properties. This method aims to solve problems of leaf spot and dry seedling diseases that have affected the quantity and quality of betel nut production. The research also includes finding ways to increase the production of important substances, quercitrin and rutin, in betel nut plants by using stimulants. The use of a hydroponics system for betel nut cultivation has also proven effective in producing fast-growing, quality plants.