A study has been conducted to understand the genetic basis of olive oil production, with a focus on the regulation of oil biosynthesis in olive trees. The research involved a comprehensive analysis of the genome and metabolic pathways of the Leccino olive cultivar, leading to the identification of MYC2 as a key regulator of fatty acid and flavonoid synthesis. This finding is expected to revolutionize the olive oil industry by enabling the breeding of olives with superior oil profiles, meeting the growing demand for healthy fats. The high-quality genome assembly generated from this research is also a valuable tool for future studies on disease resistance, environmental resilience, and sustainable farming practices in olives.