Colombia: New technique would allow to modify genes of plants and crops

Published 2021년 8월 13일

Tridge summary

Researchers have used the CRISPR / CAS9 tool to modify two genes in sugarcane with the goal of creating more productive and pesticide-resistant crops. The guide RNA molecule was used to direct the Cas9 protein to specific genes to make the desired changes. The research focused on inactivating the BU1 gene and modifying the ALS gene in a specific sugarcane variety. The BU1 gene affects the arrangement of leaves, which in turn affects solar energy capture for photosynthesis, and the ALS gene plays a role in plant resistance. This gene editing approach could be used to develop sugarcane crops that are more efficient in nitrogen uptake and more resistant to pests, diseases, and extreme climates.

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Original content

Through the CRISPR / CAS9 tool, which allows altering genes of an organism to obtain a particular benefit, two genes of the sugarcane crop were modified to generate more productive crops with greater resistance to pesticides. According to an article published by Virtual Pro, this system uses a molecule known as guide RNA (gRNA), which guides the molecular protein (Cas9) to specific areas to cut, modify or correct a gene or sequence of interest. From there, it acquires a loss or gain of function depending on the purpose. Biologist Claudia Marcela Franco, Master in Biological Sciences from the National University of Colombia (UNAL) Palmira Headquarters, author of the research Genetic editing of the sugarcane variety UFCP 82-1655 to inactivate the BU1 gene and modify the function of the ALS gene through CRISPR / CAS9 pointed out that they saw the potential of this procedure in the plants, and they set themselves the objective of contributing to the conventional improvement of the ...

Source: Redagricola