A study conducted by Italian and Spanish researchers, led by veterinarian Javier Bezos from the Complutense University of Madrid, has discovered a method to diagnose bovine tuberculosis (BTB) in Mediterranean buffaloes using the IFN-γ assay and by measuring the levels of 14 key cytokines in response to an antigen. The study, which involved 36 buffaloes, found that infected animals, whether showing symptoms or not, released higher levels of 7 cytokines compared to healthy animals. Additionally, infected animals showed increased levels of IL-1β and IP-10, while affected animals only showed increased levels of MIP-1α. The study suggests that IL-10, TNF, IL-1α, IL-6, and MIP-1β could be potential biomarkers for BTB in Mediterranean buffaloes, potentially improving the species' diagnostic yield for the disease.