Chile has detected the H5N1 avian influenza virus in the Valparaiso region, prompting protective measures and affecting poultry production. The disease, introduced via pelicans, was previously eradicated in Chile in early 2020. No human infections have been reported in Chile, but an rare case has been confirmed in a 9-year-old girl in Ecuador, marking the first in South America. The virus has been spreading across North and Central America, leading to massive culls of commercial birds and wildlife, with Chile and Ecuador being the latest additions to the list of affected countries.