Crop rotation is an effective method used in regenerative agriculture to minimize soil pathogens and promote beneficial microbial communities. Bacillus spp. and Pseudomonas species are bacteria that can inhibit the growth of plant pathogens and improve potato nutrition through the production of antibiotics and siderophores. By studying microbial diversity in soils within a crop rotation system, there is potential to identify and commercialize bacteria that can effectively protect potatoes from diseases.