A trial is underway to test 25 alfalfa ecotypes for their performance under water stress conditions in the Mediterranean dry land of Chile, as part of a project funded by the Global Crop Diversity Trust. The ecotypes being evaluated are short dormancy, grazing resistant, cold and soil salinity resistant. The first stage involved selecting the best gemplasms based on forage production and yields in a Chilean context. The trial is also being planted on farms in the Maule and Ñuble regions for validation. The project aims to provide a productive impact on the forage chain and make a scientific contribution to alfalfa improvement programs.