Researchers have discovered significant differences in the nucleolus organizing region (NOR) of American and Chinese chestnuts, impacting hybridization efforts to confer blight resistance to American chestnuts. The structure and composition of DNA in the NOR region of the Chinese chestnut differs from that of American chestnut, revealing a challenging chromosome compatibility issue between the two species. To further understand these genetic differences, researchers will use oligo-FISH techniques to study hybrids and identify parental gene origins.