The population of Botrytis cinerea in strawberry-producing areas in China are showing increasing resistance to pyraclostrobin fungicides. A survey of 206 isolates collected between 2024 and 2025 in Xinxiang confirmed resistance in 100% of the samples. The average level of resistance increased during the period. EC50 values rose from 75,31 μg/mL to 85,08 μg/mL. The number of isolates with high resistance also increased. Molecular analysis identified the G143A mutation in the BcCytb gene as the main mechanism. The gene lost an intron. This event led to the substitution of glycine for alanine at the site of action. The alteration reduced the fungicide's binding affinity to the mitochondrial target. Molecular modeling confirmed a decrease in binding energy. Resistant isolates also showed increased expression of the BcCytb gene. Expression exceeded levels observed in susceptible isolates by up to 8,5 times. This increase occurred even without exposure to the fungicide. This pattern ...